In-Silico characterization and screening of Stem Rust Resistance Gene (Sr26) from different Wheat Commercial Cultivars

Ayesha Maqsood¹, Umaira Yameen¹, Saddam Hussain¹, Nasir Ali², Nabila Farha³, Shahzad Bashir¹

1. School of Biochemistry, Minhaj University, Lahore, Pakistan.
2. Department of Chemistry, COMSAT University Islamabad, Lahore Campus, Pakistan.
3. IMBB, University of Lahore

Abstract

Wheat is most grown crop. In Pakistan wheat is the major food crop and almost 80 percent farmers grow this crop every year. As the population increases day by day and consumption of wheat also enhance and as a result its productivity rate also increases. The cause of its low productivity is the attack of stem rust diseases to wheat crop. The stem rust resistance gene (Sr26) offer resistance to disease.  The gene was amplified by using PCR technique then Gel Electrophoresis is running which show band patterns of this gene. Unfortunately the top commercial cultivars of wheat in Pakistan lack this gene. Secondly the function was predicted by different bioinformatics tools. These tools suggested that Sr26 protein have 935 amino acids with molecular weight of 105.6 kDa. This protein is rich in Leu and not stable in eukaryotic cells. The interproscan suggest this protein belong to the disease resistance protein as it contains LRR and ARC domain. This gene has the potential to be used in genetic engineering for crop improvement.

Keywords: Sr26, PCR, Gel Electrophoresis.